Recombinant FGF-2 TOP® (Thermostability Optimized)
247,00 € – 729,00 € HT
FGF-2 TOP® (formerly STAB) is a stabilized growth factor that offers a novel way to grow FGF-2 dependent cell cultures more efficiently, with fewer media changes. FGF-2 TOP® retains full biological activity even after twenty days. The stable level of FGF-2 in culture allows for a more homogenous, undifferentiated stem cell culture, while saving researchers valuable time and money because repeated supplementation with FGF-2 and a daily medium change is not required.
Core Biogenesis FGF-2 TOP® is the 155 aa mature domain of FGF-2, tag-free with nine amino acid substitutions to enhance stability without impacting bioactivity developed by Dvorak et al. 2018. This increases the functional half-life of the protein from <10 h (wild-type) to >7 days (FGF-2 TOP®) in cell culture conditions at 37ºC.
Superior thermostability:
FGF-2 TOP® is a stabilized growth factor that offers a novel way to grow FGF-2 dependent cell cultures more efficiently, with fewer media changes. FGF-2 TOP® retains full biological activity even after twenty days at 37°C. The stable level of FGF-2 in culture allows for a more homogenous, undifferentiated pluripotent stem cell cultures, and consistent downstream differentiations, while saving researchers valuable time and money because repeated supplementation with FGF-2 and a daily medium change is not required.
Superior bioactivity:
FGF-2 TOP® (Thermostability Optimized) has an expected ED50 of less than 0.1 ng/mL, as determined by NIH-3T3 cell proliferation assay. The ED50 value of FGF-2 TOP® is about 5-times lower than that of the wild-type FGF-2, as determined by a NIH/3T3 proliferation assay. Under the same experimental conditions, less FGF-2 TOP® is needed to achieve a given effect on cell proliferation, thanks to the higher stability and prolonged half-life of FGF-2 TOP®. Thanks to this competitive advantage, lower dosage of FGF-2 are required in your cell culture applications (up to 10x).
Details
50µg, 1000µg. Core Biogenesis is unlocking the powers of the bioeconomy by ensuring the scalability of world changing technologies as regenerative medicine and cellular agriculture. Contact us directly for lot reservation and (large/bulk/industry/production) quantities.
FGF-2 TOP® is produced in plant seeds of Camelina sativa cultivated in our facility in Strasbourg, France.
Engineered sequence. Neutral species. Reactivity with Human, Bovine and Porcine.
≥ 90% measured by SDS page, and resolved under reduced (R) conditions.
The biological activity of recombinant FGF-2 TOP® was determined by its ability to promote the proliferation of NIH/3T3 cells cultured in adherent condition. Cell proliferation is measured using non-radioactive colorimetric assay and monitored by absorbance. EC50 is generally: ≤ 1 ng/mL
Recombinant protein expressed in plant system, free of bacterial endotoxins.
Animal-derived Component Free. Core Biogenesis strictly guarantees that our recombinant proteins are not produced with or contain any components of animal origin.
PBS at 1mg/mL or Lyophilized.
It is recommended to further dilute the protein in sterile water or PBS to at least a concentration between 0.05-0.1 mg/ml. For specific dilutions, aliquoting and addition of carrier protein please refer to product’s data sheet or get in touch with our technical support team.
The protein is shipped to remain stable upon arrival. Once it is reconstituted can be stored at -20ºC or -80ºC.
FGF-2 TOP® (Thermostability Optimized) can be substituted directly for wild-type FGF-2 in most applications, and can be used in a wide variety of areas for cell and molecular biology. We provide high purity and bioactive protein with outstanding performance in:
- Culture and long-term expansion of pluripotent stem cells (hESCs and iPSCs).
- Growth stimulation for endothelial cells and mesenchymal stem cells (MSCs).
- Neural differentiation (from PSCs), and chondrogenic differentiation (from MSCs).
- Culture of muscle satellite Cells, and proliferating myoblasts.
- FGF2-G3 was developed by Dvorak et al., 2018. Biotechnology and Bioengineering
- B8 media protocol established by Kuo et al., 2020. Stem Cell Reports
- updated in Lyra-Leite et al., 2021. STAR Protocols
- Review of FGF 2 stabilization by Benington et al., 2020. Pharmaceutics
- Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat, Kaplan et al., 2021.
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