Recombinant IGF-1 LR3
67,00 € – 187,00 € HT
Insulin-like growth factors (IGFs) are similar to insulin by structure and function, but have a much higher growth-promoting activity. They are required for embryonic development, and are produced mainly in the liver in response to a hepatocyte growth hormone. It can be used to substitute insulin for the maintenance of human pluripotent stem cells. IGF-1 LR3 is a recombinant synthetic growth factor of ~9.1 kDa, single polypeptide chain containing 83 amino acid residues. It is an analog of IGF-1 comprised of the complete IGF-1 sequence, with an Arginine substitution for the third position Glutamic acid, and a 13 amino acid length N terminus peptide extension. It has been engineered for higher biological potency in vitro, with an increased half-life.
This recombinant IGF-1 LR3 product is a highly potent growth factor ideal for stem cell research and cell manufacturing applications, including cells of the ectoderm linage (oligodendrocytes, motor neurons), and mesoderm linage (osteocytes, chondrocytes and muscle cells).
Details
50µg, 1000µg. Core Biogenesis is unlocking the powers of the bioeconomy by ensuring the scalability of world changing technologies as regenerative medicine and cellular agriculture. Contact us directly for lot reservation and (large/bulk/industry/production) quantities.
Plant-based.
Engineered protein sequence.
≥ 95% measured by SDS page, and resolved under reduced (R) conditions.
The biological activity of recombinant IGF-1 LR3 protein was determined by its ability to promote the proliferation of cells cultured in adherent condition. Cell proliferation is measured using non-radioactive colorimetric assay and monitored by absorbance. EC50 is generally: ≤ 0.5 ng/mL
Recombinant protein expressed in plant system, free of bacterial endotoxins.
Animal-derived Component Free. Core Biogenesis strictly guarantees that our recombinant proteins are not produced with or contain any components of animal origin.
Lyophilized.
It is recommended to reconstitute the protein in sterile water or PBS to at least a concentration between 0.05-0.1 mg/ml. For specific dilutions, aliquoting and addition of carrier protein please refer to product’s data sheet or get in touch with our technical support team.
The protein is shipped to remain stable upon arrival. Once it is reconstituted can be stored at -20ºC or -80ºC.
IGF-1 LR3 can be used in a wide variety of areas for cell and molecular biology. We provide high purity animal-free, and bioactive protein with outstanding performance in: – Cell culture of muscle, bone and cartilage tissue. – Expansion of Mesenchymal Stem Cells (MSCs). -Regulation and proliferation of neural stem cells (NSCs). – Maintenance and differentiation of hematopoietic stem cells (HSCs).
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