Recombinant Bovine FGF-2

197,00 597,00  HT

FGF-2, also known as basicFGF, is a growth factor that is produced mainly in fibroblasts, endothelial cells and smooth muscle cells. FGF-2 controls fundamental biological processes, including cell growth and differentiation, tissue formation, angiogenesis and wound healing. Recombinant bovine FGF-2 consists of 146 amino acids, and a predicted molecular mass of 16.4 kDa. FGF-2 bovine is qualified for different applications including stem cell-based processes, industrial cellular agriculture, and cultivated meat production

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Details

Accession Number: P03969
Quantity:

50µg, 1000µg. Core Biogenesis is unlocking the powers of the bioeconomy by ensuring the scalability of world changing technologies as regenerative medicine and cellular agriculture. Contact us directly for lot reservation and (large/bulk/industry/production) quantities.

Origin:

FGF-2 is produced in plant seeds of Camelina sativa bred in our laboratories in Strasbourg, France.

Species:

Bovine. Similarity: -Porcine 100% -Human 99%

Purity:

≥ 80% measured by SDS page, and resolved and resolved under reduced (R) conditions. 

Bioactivity:

The biological activity of bovine recombinant FGF-2 protein was determined by its ability to promote the proliferation of NIH/3T3 cells cultured in adherent condition. Cell proliferation is measured using non-radioactive colorimetric assay and monitored by absorbance. EC50 is generally: ≤ 0.5 ng/mL

Endotoxin Level:

Recombinant protein expressed in plant system, free of bacterial endotoxins.

Animal Component-Free:

Animal-derived Component Free. Core Biogenesis strictly guarantees that our recombinant proteins are not produced with or contain any components of animal origin.

Formulation:

PBS at 1mg/mL or Lyophilized

Preparation:

It is recommended to further dilute the protein in sterile water or PBS to at least a concentration between 0.05-0.1 mg/ml. For specific dilutions, aliquoting and addition of carrier protein please refer to product’s data sheet or get in touch with our technical support team.

Storage:

The protein is shipped to remain stable upon arrival. Once it is reconstituted can be stored at -20ºC or -80ºC.

Featured Application Areas:

Bovine FGF-2 can be used in a wide variety of areas for cell and molecular biology. We provide high purity and biocative protein with outstanding performance in: – Culture and long-term expansion of pluripotent stem cells (iPSCs). – Growth stimulation for endothelial cells and mesenchymal stem cells (MSCs). – Chondrogenic differentiation (from MSCs). – Culture of Bovine Satellite Cells (BSCs) and proliferating myoblasts.

Publications:

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