Human FGF-2

FGF-2, also known as basicFGF, is a growth factor that is produced mainly in fibroblasts, endothelial cells and smooth muscle cells. FGF-2 controls fundamental biological processes, including cell growth and differentiation, tissue formation, angiogenesis and wound healing. Recombinant human FGF-2 consists of 146 amino acids,16.06 kDa. FGF-2 is qualified for different applications including industrial cell-based meat production or cell culture.

Details

Accession Number: P09038
Quantity:

25ug,50ug, 100ug, 500ug, 1000ug. Core Biogenesis is unlocking the powers of the bioeconomy by ensuring the scalability of world changing technologies as regenerative medicine and cellular agriculture. Contact us directly for lot reservation and (large/bulk/industry/production) quantities.

Origin:

FGF-2 is produced in plant seeds of Camelina sativa bred in our laboratories in Strasbourg, France.

Species:

Human. Similarity: -Bovine 99% -Porcine 99% -Mouse 94%

Purity:

≥ 95% measured by SDS page, and resolved under reduced (R) and non-reduced (NR) conditions.

Bioactivity:

The biological activity of human recombinant FGF-2 protein was determined by its ability to promote the proliferation of 3T3 cells cultured in adherent condition. Cell proliferation was measured using non-radioactive colorimetric assay and monitored by absorbance. ED50 < 0.1 ng/ml.

Endotoxin Level:

Low endotoxin level ≤ 0.005ng/µg (≤ 0.005EU/ug of protein) as determined by Limulus Amebocyte Lysate assay.

Animal Component-Free:

Xeno-Free Product. Core Biogenesis strictly guarantees that our recombinant proteins have no trace of animal components.

Formulation:

Tris pH 7.4. Our products are shipped in lyophilised format to ensure protein stability in worldwide deliveries.

Preparation:

It is recommended to reconstitute the protein in sterile water to at least a concentration between 0.05-1.0 mg/ml. For dilutions, aliquoting and addition of carrier protein please refer to product’s data sheet or get in touch with our technical support team.

Storage:

Lyophilised protein is stable at room temperature upon arrival. Once it is reconstituted can be stored at -20ºC or -80ºC for up to 1 year.

Featured Application Areas:

Human FGF-2 can be used in a wide variety of areas for cell and molecular biology. We provide high purity and biocative protein with outstanding performance in: – Culture and long-term expansion of pluripotent stem cells (hESCs and iPSCs). – Growth stimulation for endothelial cells and mesenchymal stem cells (MSCs). – Neural differentiation (from PSCs), and Chondrogenic differentiation (from MSCs). – Culture of Bovine Satellite Cells (BSCs) and proliferating myoblasts.

Publications:

Coming soon! Get in touch if you want to become part of our customer spotlight and get special support for your next paper.

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