Human EGF

EGF, is a growth factor that is produced mainly in platelets, macrophages and fibroblasts. EGF controls fundamental biological processes, including cell growth, differentiation and survival. Its mitogenic effect includes stimulation of hair growth and human skin fibroblast proliferation. Recombinant EGF human consists of 53 amino acids, 5.83 kDa. Core EGF is qualified for different applications including industrial gastrointestinal treatment, wound healing or cell culture.

Details

Accession Number: P01133
Quantity:

100ug, 500ug, 1000ug. Core Biogenesis is unlocking the powers of the bioeconomy by ensuring the scalability of world changing technologies as regenerative medicine and cellular agriculture. Contact us directly for lot reservation and (large/bulk/industry/production) quantities. “

Origin:

EGF is produced in plant seeds of Camelina sativa bred in our laboratories in Strasbourg, France.

Species:

Human. Similarity: -Porcine 85% -Mouse 74% -Bovine 70%

Purity:

≥ 95% measured by SDS page, and resolved under reduced (R) and non-reduced (NR) conditions.

Bioactivity:

The biological activity of human recombinant EGF protein was determined by its ability to promote the proliferation of 3T3 cells cultured in adherent condition. Cell proliferation was measured using non-radioactive colorimetric assay and monitored by absorbance. ED50 < 0.1 ng/ml.

Endotoxin Level:

Low endotoxin level ≤ 0.005ng/µg (≤ 0.005EU/ug of protein) as determined by Limulus Amebocyte Lysate assay.

Animal Component-Free:

Xeno-Free Product. Core Biogenesis strictly guarantees that our recombinant proteins have no trace of animal components.

Formulation:

Tris pH 7.4. Our products are shipped in lyophilised format to ensure protein stability in worldwide deliveries.

Preparation:

It is recommended to reconstitute the protein in sterile water to at least a concentration between 0.05-1.0 mg/ml. For dilutions, aliquoting and addition of carrier protein please refer to product’s data sheet or get in touch with our technical support team.

Storage:

Lyophilised protein is stable at room temperature upon arrival. Once it is reconstituted can be stored at -20ºC or -80ºC for up to 1 year.

Featured Application Areas:

Human EGF can be used in a wide variety of areas for cell and molecular biology. We provide high purity and bioactive protein with outstanding performance in: – Culture of pluripotent stem cells and differentiation into ectoderm linage (including epithelial cells and neural progenitors) and endoderm linage ( multipotent progenitors and hepatocytes). – Induction of intestinal (healthy and cancer) organoids.

Publications:

Coming soon! Get in touch if you want to become part of our customer spotlight and get special support for your next paper.

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