Recombinant EGF
67,00 € – 187,00 € HT
Epidermal Growth Factor (EGF) is a potent mitogenic growth factor ~6 kDa protein, consisting of a single polypeptide of 53 amino acid residues and three intramolecular disulfide bonds that stimulates cell growth, proliferation and differentiation, oncogenesis and wound healing. EGF exerts its effects in the target cells by binding to the transmembrane protein tyrosine kinase EGF receptor. EGF proteins are evolutionarily conserved across many species.
This recombinant EGF product is a highly pure and bioactive growth factor providing high performance and reproducible results among various stem cell culture, and cell manufacturing applications, including direct differentiation of hepatic progenitors into mature hepatocytes, expansion of neural progenitors, formation of germ cells, formation of intestinal organoids, as well as in wound healing studies, tissue engineering, and cancer research.
Details
50µg, 1000µg. Core Biogenesis is unlocking the powers of the bioeconomy by ensuring the scalability of world changing technologies as regenerative medicine and cellular agriculture. Contact us directly for lot reservation and (large/bulk/industry/production) quantities.
Plant-derived.
Recombinant protein.
≥ 95% measured by SDS page, and resolved under reduced (R) conditions.
The biological activity of recombinant EGF protein was determined by its ability to promote the proliferation of Balb/3T3 cells cultured in adherent condition. Cell proliferation is measured using non-radioactive colorimetric assay and monitored by absorbance. EC50 is generally: ≤ 0.5 ng/mL
Recombinant protein expressed in plant system, free of bacterial endotoxins.
Animal-derived Component Free. Core Biogenesis strictly guarantees that our recombinant proteins are not produced with or contain any components of animal origin.
Lyophilized
It is recommended to reconstitute the protein in sterile water or PBS to at least a concentration between 0.05-0.1 mg/ml. For specific dilutions, aliquoting and addition of carrier protein please refer to product’s data sheet or get in touch with our technical support team.
The protein is shipped to remain stable upon arrival. Once it is reconstituted can be stored at -20ºC or -80ºC.
Recombinant EGF can be used in a wide variety of areas for cell and molecular biology. We provide high purity and bioactive protein with outstanding performance in: – Culture of pluripotent stem cells and differentiation into ectoderm linage (including epithelial cells and neural progenitors) and endoderm linage ( multipotent progenitors and hepatocytes). – Induction of intestinal (healthy and cancer) organoids. – Development of complex wound healing and epithelial tissue models.
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