Recombinant Human FGF-2
197,00 € – 597,00 € HT
FGF-2, also known as basicFGF, is a growth factor that is produced mainly in fibroblasts, endothelial cells and smooth muscle cells. FGF-2 controls fundamental biological processes, including cell growth and differentiation, tissue formation, angiogenesis and wound healing. FGF-2 is a critical component of advanced stem cell culture models, such as iPSCs and hESCs. Recombinant human FGF-2 consists of 146 amino acids, with a predicted molecular mass of 16.06 kDa.
Human FGF-2 is qualified for different applications including stem cell research, organoid development, tissue engineering, and cell-based manufactruing technologies.
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Details
50µg, 1000µg. Core Biogenesis is unlocking the powers of the bioeconomy by ensuring the scalability of world changing technologies as regenerative medicine and cellular agriculture. Contact us directly for lot reservation and (large/bulk/industry/production) quantities.
Human FGF-2 is produced in plant seeds of Camelina sativa bred in our laboratories in Strasbourg, France.
Human. Similarity: -Bovine 99% -Porcine 99% -Mouse 94%
≥ 90% measured by SDS page, and resolved under reduced (R) conditions.
The biological activity of human recombinant FGF-2 protein was determined by its ability to promote the proliferation of NIH/3T3 cells cultured in adherent condition. Cell proliferation is measured using non-radioactive colorimetric assay and monitored by absorbance. EC50 is generally: ≤ 0.5 ng/mL.
Recombinant protein expressed in plant system, free of bacterial endotoxins.
Animal-derived Component Free. Core Biogenesis strictly guarantees that our recombinant proteins are not produced with or contain any components of animal origin.
PBS at 1mg/mL or Lyophilized.
It is recommended to further dilute the protein in sterile water or PBS to at least a concentration between 0.05-0.1 mg/ml. For specific dilutions, aliquoting and addition of carrier protein please refer to product’s data sheet or get in touch with our technical support team.
The protein is shipped to remain stable upon arrival. Once it is reconstituted can be stored at -20ºC or -80ºC.
Human FGF-2 can be used in a wide variety of areas for cell and molecular biology. We provide high purity and biocative protein with outstanding performance in: – Culture and long-term expansion of pluripotent stem cells (hESCs and iPSCs). – Growth stimulation for endothelial cells and mesenchymal stem cells (MSCs). – Neural differentiation (from PSCs), and Chondrogenic differentiation (from MSCs). – Culture of Muscle Satellite Cells, and proliferating myoblasts.
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