Core Biogenesis

Technology

Molecular Farming is the use of entire plants as expression hosts for the production of recombinant proteins. DNA is inserted into the plant in a transient or stable manner, and this DNA is then used as the code for the plant to synthesize the corresponding proteins, in our case Growth Factors and cytokines.

The protein of interest accumulates in the plant and must then be extracted and purified in order to obtain the pure final product.

Our biofactory plant, camelina sativa.

Core Biogenesis leverages an oilseed plant called Camelina sativa for the stable expression of recombinant proteins and has developed various technologies to increase the expression yield of its plants and make the extraction process more efficient and more scalable. 

In addition to scalability and cost-effectiveness, molecular farming using a stable expression method has substantial sustainability benefits: our method at Core Biogenesis doesn’t require bioreactors, nor automated vertical farming technologies, allowing for a carbon-negative production thanks to the photosynthesis mechanism of the plant. 

Overview of Core Biogenesis production process and proprietary technologies.

Solving the 2 key bottlenecks in bioproduction

The expression yield

Leveraging the expertise of its CSO, Dr. Chouaib Meziadi, in plant genetics and epigenetics, Core Biogenesis has identified various combinations of genes in Camelina Sativa, that are  involved in plant defense mechanisms and that increase the expression yield of the recombinant protein expressed in the plant, by orders of magnitude.


1.Control

2. Conventional technology

3. Core Biogenesis technology

Increased protein expression with Core Biogenesis technology vs conventional expression methods: 

1: Untransformed C.sativa leaf (no detection).
2: C. sativa leaves expressing RFP (Red Fluorescent Protein) without Core Biogenesis technology.
3: C. sativa leaves expressing RFP (Red Fluorescent Protein) with Core Biogenesis technology.

All three leaves were exposed to 558 nm excitation wave for RFP (Red Fluorescent Protein) revelation and under normal light. 

This piece of technology removes the first bottleneck which is the low expression yield.


1.Control

2. Conventional technology

3. Core Biogenesis technology

1: Untransformed C.sativa seeds (no detection).
2: C. sativa seeds expressing RFP (Red Fluorescent Protein) without Core Biogenesis technology.
3: C. sativa seeds expressing RFP (Red Fluorescent Protein) with Core Biogenesis technology.

technology

Our downstream process

The fixed cost of extraction and purification represent the true bottleneck of any bioproduction method, including molecular farming.

Core Biogenesis bioproduction method leverages the oleosin fusion technology, the targeted isolation of the recombinant protein of interest to the lipid body, a specific organelle of the plant located in the seeds of Camelina sativa, allowing for the development of phase-specific downstream process development that are scalable and guarantee a final product free from impurities.

This results in a downstream process with a scalability comparable to that of the food industry, with a cGMP quality grade. 

With these two bottlenecks (expression yield and purification) solved, Core Biogenesis unlocks the access to the true scalability of molecule farming, the use of plants for the bioproduction of recombinant proteins.

With the scalability of this technology fully unleashed, Core Biogenesis has begun open field cultivation and multiplication of its plants in early 2022, and will soon scale-up to dozens of hectares of open field surface to reach the volumes and cost-reduction needed by companies in the cell & gene therapy industry and the cellular agriculture industry, in order to help make their products mainstream and cost-competitive.