FGF-2 STAB ®

247,00 729,00  HT

FGF-2, also known as basicFGF, is a growth factor that controls fundamental biological processes and is qualified for different applications including industrial cell-based meat production, cell culture and cell therapy manufacturing.

We have licensed from Enantis a thermostable (heat stable) FGF-2 called FGF-2 STAB ® (FGF-2-G3). We use our proprietary manufacturing platform to produce FGF-2 STAB, our production process is animal-free, endotoxin-free and biorisk-free, with unparalleled scalability and cost-reduction possibilities.

Superior thermostability:

FGF-2-STAB® is a stabilized growth factor that offers a novel way to grow FGF-2-dependent cell cultures more efficiently, with fewer media changes. FGF2-STAB® retains full biological activity even after twenty days at 37°C. The stable level of FGF-2 in culture allows for a more homogenous, undifferentiated stem cell culture, while saving researchers valuable time and money because repeated supplementation with FGF-2 and a daily medium change is not required. 

Superior bioactivity:


FGF2-STAB has an expected ED50 of less than 0.1 ng/mL, as determined by NIH-3T3 cell proliferation assay. The ED50 value of FGF-2 STAB® is about 5-times lower than that of the wild-type FGF-2, as determined by a NIH/3T3 proliferation assay. Under the same experimental conditions, less FGF-2 STAB® is needed to achieve a given effect on cell proliferation, thanks to the higher stability and prolonged half-life of FGF-2 STAB®. Thanks to this competitive advantage, lower dosage of FGF-2 are required in your cell culture applications (up to 10x). 

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Details

Quantity:

5ug, 50ug, 1000ug. Bulk orders available.

Origin:

FGF-2 is produced in plant seeds of Camelina sativa cultivated in our facility in Strasbourg, France.

Purity:

≥ 95% measured by SDS page, and resolved under reduced (R) and non-reduced (NR) conditions.

Bioactivity:

The biological activity of recombinant FGF-2 STAB® was determined by its ability to promote the proliferation of 3T3 cells cultured in adherent condition. Cell proliferation was measured using non-radioactive colorimetric assay and monitored by absorbance. ED50 is < 0.1 ng/ml.

Endotoxin Level:

Low endotoxin level ≤ 0.005ng/µg (≤ 0.005EU/ug of protein) as determined by Limulus Amebocyte Lysate assay.

Animal Component-Free:

Xeno-Free Product. Core Biogenesis strictly guarantees that our recombinant proteins have no trace of animal components.

Formulation:

Our products are shipped in lyophilised format to ensure protein stability in worldwide deliveries.

Storage:

Lyophilized, to store at -20°C to -80°C, stable for up to 12 months when stored at -20°C to -80°C. Once it is reconstituted it can be stored at -20ºC or -80ºC for up to 1 year.

Featured Application Areas:

 FGF-2 STAB® can be substituted directly for wild-type FGF-2 in most applications and can be used in a wide variety of areas for cell and molecular biology. We provide high purity and bioactive protein with outstanding performance in: 

  • Culture and long-term expansion of pluripotent stem cells (hESCs and iPSCs). 
  • Growth stimulation for endothelial cells and mesenchymal stem cells (MSCs). 
  • Neural differentiation (from PSCs), and Chondrogenic differentiation (from MSCs). 
  • Culture of Bovine Satellite Cells (BSCs) and proliferating myoblasts.
Publications:
  • FGF2-G3 was developed by Dvorak et al., 2018. Biotechnology and Bioengineering 
  • B8 media protocol established by Kuo et al., 2020. Stem Cell Reports 
  • updated in Lyra-Leite et al., 2021. STAR Protocols 
  • Review of FGF 2 stabilization by Benington et al., 2020. Pharmaceutics
  • Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat, Kaplan et al., 2021.

Are you using FGF-2 for exciting research or applications?

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