FGF-2 STAB ®
97,00 € – 597,00 € HT
FGF-2, also known as basicFGF, is a growth factor that controls fundamental biological processes and is qualified for different applications including industrial cell-based meat production, cell culture and cell therapy manufacturing.
We have licensed from Enantis a thermostable (heat stable) FGF-2 called FGF-2 STAB ® (FGF-2-G3). We use our proprietary manufacturing platform to produce FGF-2 STAB, our production process is animal-free, endotoxin-free and biorisk-free, with unparalleled scalability and cost-reduction possibilities.
Superior thermostability:
FGF-2-STAB® is a stabilized growth factor that offers a novel way to grow FGF-2-dependent cell cultures more efficiently, with fewer media changes. FGF2-STAB® retains full biological activity even after twenty days at 37°C. The stable level of FGF-2 in culture allows for a more homogenous, undifferentiated stem cell culture, while saving researchers valuable time and money because repeated supplementation with FGF-2 and a daily medium change is not required.
Superior bioactivity:
FGF2-STAB has an expected ED50 of less than 0.1 ng/mL, as determined by NIH-3T3 cell proliferation assay. The ED50 value of FGF-2 STAB® is about 5-times lower than that of the wild-type FGF-2, as determined by a NIH/3T3 proliferation assay. Under the same experimental conditions, less FGF-2 STAB® is needed to achieve a given effect on cell proliferation, thanks to the higher stability and prolonged half-life of FGF-2 STAB®. Thanks to this competitive advantage, lower dosage of FGF-2 are required in your cell culture applications (up to 10x).
Details
5ug, 50ug, 1000ug. Bulk orders available.
FGF-2 is produced in plant seeds of Camelina sativa cultivated in our facility in Strasbourg, France.
≥ 95% measured by SDS page, and resolved under reduced (R) and non-reduced (NR) conditions.
The biological activity of recombinant FGF-2 STAB® was determined by its ability to promote the proliferation of 3T3 cells cultured in adherent condition. Cell proliferation was measured using non-radioactive colorimetric assay and monitored by absorbance. ED50 is < 0.1 ng/ml.
Low endotoxin level ≤ 0.005ng/µg (≤ 0.005EU/ug of protein) as determined by Limulus Amebocyte Lysate assay.
Xeno-Free Product. Core Biogenesis strictly guarantees that our recombinant proteins have no trace of animal components.
Our products are shipped in lyophilised format to ensure protein stability in worldwide deliveries.
Lyophilized, to store at -20°C to -80°C, stable for up to 12 months when stored at -20°C to -80°C. Once it is reconstituted it can be stored at -20ºC or -80ºC for up to 1 year.
FGF-2 STAB® can be substituted directly for wild-type FGF-2 in most applications and can be used in a wide variety of areas for cell and molecular biology. We provide high purity and bioactive protein with outstanding performance in:
- Culture and long-term expansion of pluripotent stem cells (hESCs and iPSCs).
- Growth stimulation for endothelial cells and mesenchymal stem cells (MSCs).
- Neural differentiation (from PSCs), and Chondrogenic differentiation (from MSCs).
- Culture of Bovine Satellite Cells (BSCs) and proliferating myoblasts.
- FGF2-G3 was developed by Dvorak et al., 2018. Biotechnology and Bioengineering
- B8 media protocol established by Kuo et al., 2020. Stem Cell Reports
- updated in Lyra-Leite et al., 2021. STAR Protocols
- Review of FGF 2 stabilization by Benington et al., 2020. Pharmaceutics
- Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat, Kaplan et al., 2021.
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